pet-22b (+) vector (Thermo Fisher)
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90
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Thermo Fisher
pet-22b (+) vector
Pet 22b (+) Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet-22b+(+)+vector/pm39818401-55-4-31
Average 90 stars, based on 1 article reviews
Pet 22b (+) Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet-22b+(+)+vector/pm39818401-55-4-31
Average 90 stars, based on 1 article reviews
pet-22b (+) vector - by Bioz Stars,
2026-10
90/100 stars
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Clone Assay:Article Title: Structural basis of the IL-1 receptor TIR domain-mediated IL-1 signaling Article Snippet: The coding sequences of following TIR domains, IL-1RAcP-TIR (residues 403–550), IL-1RAcPb-TIR (residues 403–548), IL-18Rβ-TIR (residues 406–562), IL-1RAPL2-TIR (residues 400–560), zSIGIRR-TIR (residues 180–327) and zSIGIRR-C299S-TIR (residues 180–327), were cloned into pET-22b vector (Invitrogen) with a C-terminal 6×His tag. cDNAs for human full-length IL-1RAcP (residues 1–570), IL -1RAcPb (residues 1–687) and a series of related mutants were cloned into pcDNA3.1( Article Title: Oral immunization of mice with Lactococcus lactis expressing Shiga toxin truncate confers enhanced protection against Shiga toxins of Escherichia coli O157:H7 and Shigella dysenteriae. Article Snippet: Regardless of the communal impact of shiga toxins, till today neither a specific treatment nor licensed vaccine is available.. Lactococcus lactis (L. Lactis), generally regarded as safe organism, is well known to provide a valuable approach regarding the oral delivery of vaccines.. This study was undertaken to evaluate the protective efficacy of Stx2a1 expressed in nisin inducible L. lactis, against Shiga toxins (Stx1, Stx2) in mouse model. Plasmid Preparation:Article Title: Structural basis of the IL-1 receptor TIR domain-mediated IL-1 signaling Article Snippet: The coding sequences of following TIR domains, IL-1RAcP-TIR (residues 403–550), IL-1RAcPb-TIR (residues 403–548), IL-18Rβ-TIR (residues 406–562), IL-1RAPL2-TIR (residues 400–560), zSIGIRR-TIR (residues 180–327) and zSIGIRR-C299S-TIR (residues 180–327), were cloned into pET-22b vector (Invitrogen) with a C-terminal 6×His tag. cDNAs for human full-length IL-1RAcP (residues 1–570), IL -1RAcPb (residues 1–687) and a series of related mutants were cloned into pcDNA3.1( Article Title: Oral immunization of mice with Lactococcus lactis expressing Shiga toxin truncate confers enhanced protection against Shiga toxins of Escherichia coli O157:H7 and Shigella dysenteriae. Article Snippet: Regardless of the communal impact of shiga toxins, till today neither a specific treatment nor licensed vaccine is available.. Lactococcus lactis (L. Lactis), generally regarded as safe organism, is well known to provide a valuable approach regarding the oral delivery of vaccines.. This study was undertaken to evaluate the protective efficacy of Stx2a1 expressed in nisin inducible L. lactis, against Shiga toxins (Stx1, Stx2) in mouse model. Amplification:Article Title: Structural basis of the IL-1 receptor TIR domain-mediated IL-1 signaling Article Snippet: The coding sequences of following TIR domains, IL-1RAcP-TIR (residues 403–550), IL-1RAcPb-TIR (residues 403–548), IL-18Rβ-TIR (residues 406–562), IL-1RAPL2-TIR (residues 400–560), zSIGIRR-TIR (residues 180–327) and zSIGIRR-C299S-TIR (residues 180–327), were cloned into pET-22b vector (Invitrogen) with a C-terminal 6×His tag. cDNAs for human full-length IL-1RAcP (residues 1–570), IL -1RAcPb (residues 1–687) and a series of related mutants were cloned into pcDNA3.1( Article Title: Oral immunization of mice with Lactococcus lactis expressing Shiga toxin truncate confers enhanced protection against Shiga toxins of Escherichia coli O157:H7 and Shigella dysenteriae. Article Snippet: Regardless of the communal impact of shiga toxins, till today neither a specific treatment nor licensed vaccine is available.. Lactococcus lactis (L. Lactis), generally regarded as safe organism, is well known to provide a valuable approach regarding the oral delivery of vaccines.. This study was undertaken to evaluate the protective efficacy of Stx2a1 expressed in nisin inducible L. lactis, against Shiga toxins (Stx1, Stx2) in mouse model. Polymerase Chain Reaction:Article Title: Structural basis of the IL-1 receptor TIR domain-mediated IL-1 signaling Article Snippet: The coding sequences of following TIR domains, IL-1RAcP-TIR (residues 403–550), IL-1RAcPb-TIR (residues 403–548), IL-18Rβ-TIR (residues 406–562), IL-1RAPL2-TIR (residues 400–560), zSIGIRR-TIR (residues 180–327) and zSIGIRR-C299S-TIR (residues 180–327), were cloned into pET-22b vector (Invitrogen) with a C-terminal 6×His tag. cDNAs for human full-length IL-1RAcP (residues 1–570), IL -1RAcPb (residues 1–687) and a series of related mutants were cloned into pcDNA3.1( Article Title: Oral immunization of mice with Lactococcus lactis expressing Shiga toxin truncate confers enhanced protection against Shiga toxins of Escherichia coli O157:H7 and Shigella dysenteriae. Article Snippet: Regardless of the communal impact of shiga toxins, till today neither a specific treatment nor licensed vaccine is available.. Lactococcus lactis (L. Lactis), generally regarded as safe organism, is well known to provide a valuable approach regarding the oral delivery of vaccines.. This study was undertaken to evaluate the protective efficacy of Stx2a1 expressed in nisin inducible L. lactis, against Shiga toxins (Stx1, Stx2) in mouse model. Purification:Article Title: Structural basis of the IL-1 receptor TIR domain-mediated IL-1 signaling Article Snippet: The coding sequences of following TIR domains, IL-1RAcP-TIR (residues 403–550), IL-1RAcPb-TIR (residues 403–548), IL-18Rβ-TIR (residues 406–562), IL-1RAPL2-TIR (residues 400–560), zSIGIRR-TIR (residues 180–327) and zSIGIRR-C299S-TIR (residues 180–327), were cloned into pET-22b vector (Invitrogen) with a C-terminal 6×His tag. cDNAs for human full-length IL-1RAcP (residues 1–570), IL -1RAcPb (residues 1–687) and a series of related mutants were cloned into pcDNA3.1( Article Title: Oral immunization of mice with Lactococcus lactis expressing Shiga toxin truncate confers enhanced protection against Shiga toxins of Escherichia coli O157:H7 and Shigella dysenteriae. Article Snippet: Regardless of the communal impact of shiga toxins, till today neither a specific treatment nor licensed vaccine is available.. Lactococcus lactis (L. Lactis), generally regarded as safe organism, is well known to provide a valuable approach regarding the oral delivery of vaccines.. This study was undertaken to evaluate the protective efficacy of Stx2a1 expressed in nisin inducible L. lactis, against Shiga toxins (Stx1, Stx2) in mouse model. Transformation Assay:Article Title: Structural basis of the IL-1 receptor TIR domain-mediated IL-1 signaling Article Snippet: The coding sequences of following TIR domains, IL-1RAcP-TIR (residues 403–550), IL-1RAcPb-TIR (residues 403–548), IL-18Rβ-TIR (residues 406–562), IL-1RAPL2-TIR (residues 400–560), zSIGIRR-TIR (residues 180–327) and zSIGIRR-C299S-TIR (residues 180–327), were cloned into pET-22b vector (Invitrogen) with a C-terminal 6×His tag. cDNAs for human full-length IL-1RAcP (residues 1–570), IL -1RAcPb (residues 1–687) and a series of related mutants were cloned into pcDNA3.1( Article Title: Oral immunization of mice with Lactococcus lactis expressing Shiga toxin truncate confers enhanced protection against Shiga toxins of Escherichia coli O157:H7 and Shigella dysenteriae. Article Snippet: Regardless of the communal impact of shiga toxins, till today neither a specific treatment nor licensed vaccine is available.. Lactococcus lactis (L. Lactis), generally regarded as safe organism, is well known to provide a valuable approach regarding the oral delivery of vaccines.. This study was undertaken to evaluate the protective efficacy of Stx2a1 expressed in nisin inducible L. lactis, against Shiga toxins (Stx1, Stx2) in mouse model. |